Microbiological Quality Assessment of Some Brands of Cosmetic Hair Care Preparations Sold Within Alkhoms City, Libya

تاريخ الاستلام: 14-4-2021م

تاريخ التقييم: 26-5-2020م

Pages:320-326

Gamal M. A. B (1) , Abo Azza M. M. (2) , Al Gayeed A. O. A (3) And shakurfow F. A. A. (4)

الملخص:

الملخص: من لحظة فتح منتج الشامبو حتى يتخلص منه المستهلك، فإنه يخضع لتلوث جرثومي مستمر ومتغير من البيئة المحلية وأيدي المستهلكين وسوائل أجسامهم. المواد: تم شراء ما مجموعه 18 عينة تمثيلية في أماكن البيع بالتجزئة بمدينة الخمس، ليبيا. وكانت هذه العينات عبارة عن شامبو للشعر وكحل متوفر حاليا في الأسواق المحلية. الوسائط المستخدمة: أجار المغذيات، أجار الدم، أجار ملح المانيتول، ماء الببتون، أجار مستخلص خميرة التربتون تم استخدامها في عزل وتحديد الحمل البكتيري للعينة. تم استخدام Sabouraud dextrose agar لعزل وتعداد الخمائر. الطريقة: تم تحديد التعداد البكتيريولوجي لشامبو مستحضرات التجميل والعزلات الفطرية بناءً على مظهرها المجهري. النتائج: العلامتان التجـــــاريتــــــان همــــــــــا Dove و Pantene، من 22.2٪ من Dove shampoo S. تم عزل البشرة بمعدل أقل من 1000 cfu / g. و 11.1٪ تحتوي على Rhizopus spp. تشمل العلامتان التجاريتان زيوت الشعر Parachute و Good Life. من 22.2٪ من زيت شعر المظلة B. subtilis تم عزلها بمعدل 100 cfu / g. لم يتم اكتشاف بكتيريا أو عفن أو خمائر أخرى في العينات المختبرة. كما لوحظت نفس النتائج مع زيت الشعر Good life ولكن تم عزل B. subtilis من 11.1٪ من العينات التي تم فحصها. الإستنتاج: كانت جميع العينات المختبرة لمستحضرات التجميل للشعر بما في ذلك الشامبو والزيوت خالية من التلوث البكتيري اللاهوائي.

Abstract:

From the moment of shampoo product is opened until the consumer discards it, it is subject to constant and variable microbial contamination from the domestic environment and the consumers’ hands and body fluids. Materials: A total of 18 representative samples were purchased at retail outlets in AlKhoms, Libya. These samples were hair shampoo and kohl, which are currently available in the local markets. Media used: Nutrient agar, blood agar, mannitol salt agar, peptone water and tryptone yeast extract agar were used in the isolation and determination of the bacterial load of the sample. Sabouraud dextrose agar was used for the isolation and enumeration of yeasts and molds. Method: Bacteriological counts of the cosmetic shampoo, fungal isolates were identified based on their macroscopic and microscopic appearance. Results: The two brands include Dove and Pantene shampoos, From 22.2% of Dove shampoo S. epidermidis was isolated at rate of less than 1000 cfu/g. and 11.1% contain Rhizopus spp. The two brands include Parachute and Good life hair oils. From 22.2% of Parachute hair oil B. subtilis was isolated at rate of 100 cfu/g. No other bacteria, molds or yeasts detected in the tested samples. Also the same results observed with Good life hair oil but B. subtilis was isolated from 11.1% of the examined samples. Summary: Two brands of hair shampoos were tested for their total aerobic bacterial, coliform and fungal counts. Only Staphylococcus epidermidis and Rhizopus spp. were detected. However Bacillus subtilis was isolated from 11.1% of the tested hair oil samples. All tested samples of cosmetic hair preparations including hair shampoos and oils were free from anaerobic bacterial contamination.
Keywords: shampoo, oil, contamination

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