218925167620+ / 218919656575+ / 218916307390+ / 218911653137+
95 / 2020
دار الكتب الوطنية بنغازي
Protective Effects of Honey on Carbon Tetrachloride Induced Hepatotoxicity in Rats
تاريخ الاستلام: 15-10-2020م
تاريخ التقييم: 25-10-2020م
Pages:1-17
Mahmoud Mohamed Howas - and Mohamed Ahmed Amer
تهدف الدراسة الي تقدير التاثيرات السميةعلي الكبد لرابع كلوريد الكربون والتأثيرات الوقائية لعسل النحل ضد هذه السمية و ذلك باختبارها علي الفئران المعملية من نوف ويسترحيث اجريت الدراسة علي اربع مجموهات منها. تم تنشيط هذه الافة بحقنة داخل الصفاق باستخدام محلول مخفف من رابع كلوريد الكربون في زيت الزيتون ( 0.5 مل /كجم من وزن الجسم ) و بنسبة 1:1 , و تم الحقن مرتين في الاسبوع لمدة اربع اسابيع. تم بعد ذلك تقديرالضررباستخدام مجموعة من المقاييس الحيوية مثل AST, ALT, LDH , CAT . بعد الحقن برابع كلوريد الكربون لمدة اربع اسابيع لوحظت زيادة كبيرة في مستويات AST , ALT , LDH مقارنة بالمجوعة القياسية الاولي G1, بينما قلت بوضوح مستويات هذه الانزيمات عند الحقن بخليط رابع كلوريد الكربون مع العسل (10% ) لمدة اربع اسابيع مقارنة بالمجموعة الثالثة G3 (P < 0.05) , و لوحظ ايضا نقص واضح في قيمة CAT للمجموعة الثالثة مقارنة مه المجموعة الاولي G1 .
The present paper aimed to evaluate the hepatotoxic effects of carbon tetrachloride and the hepatoprotective effects of bees’ honey on rats, the experiment was carried out on four groups of female Wister rats. Hepatic lesions were induced by intraperitoneal (i.p) injection of carbon tetrachloride (0.5 ml/Kg body weight (b.wt)) diluted in olive oil (0.5 ml/Kg with 1:1 ratio), twice a week a period of for four weeks. Biochemical parameters of hepatic damage such as serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and catalase (CAT) activity were determined. Four weeks treatment using Carbon tetrachloride increased the serum AST, ALT and LDH levels significantly compared to control group (G1), while these enzymes were significantly reduced by the effect of honey (10%) plus CCl4 (0.5ml/Kg (b.wt) (i.p.)) for 4 weeks compared to CCl4 group (G3) (P< 0.05),
but there were significant decreases in catalase (CAT) values of CCl4 group (G3) compared to the control group (G1) (P< 0.05).
Keywords: Liver, hepatotoxic , Free radical, Antioxidation.
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