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رقم الإيداع المحلي
95 / 2020
دار الكتب الوطنية بنغازي
ISSN: 2706-9087
المجلد الخامس
العدد العاشر لشهر ديسمبر 2020

رجوع

Protective Effects of Honey on Carbon Tetrachloride Induced Hepatotoxicity in Rats

تاريخ الاستلام: 15-10-2020م

تاريخ التقييم: 25-10-2020م

Pages:1-17

Mahmoud Mohamed Howas - and Mohamed Ahmed Amer
الملخص:

تهدف الدراسة الي تقدير التاثيرات السميةعلي الكبد لرابع كلوريد الكربون والتأثيرات الوقائية لعسل النحل ضد هذه السمية و ذلك باختبارها علي الفئران المعملية من نوف ويسترحيث اجريت الدراسة علي اربع مجموهات منها. تم تنشيط هذه الافة بحقنة داخل الصفاق باستخدام محلول مخفف من رابع كلوريد الكربون في زيت الزيتون ( 0.5 مل /كجم من وزن الجسم ) و بنسبة 1:1 , و تم الحقن مرتين في الاسبوع لمدة اربع اسابيع. تم بعد ذلك تقديرالضررباستخدام مجموعة من المقاييس الحيوية مثل AST, ALT, LDH , CAT . بعد الحقن برابع كلوريد الكربون لمدة اربع اسابيع لوحظت زيادة كبيرة في مستويات AST , ALT , LDH مقارنة بالمجوعة القياسية الاولي G1, بينما قلت بوضوح مستويات هذه الانزيمات عند الحقن بخليط رابع كلوريد الكربون مع العسل (10% ) لمدة اربع اسابيع مقارنة بالمجموعة الثالثة G3 (P < 0.05) , و لوحظ ايضا نقص واضح في قيمة CAT للمجموعة الثالثة مقارنة مه المجموعة الاولي G1 .

Abstract:

The present paper aimed to evaluate the hepatotoxic effects of carbon tetrachloride and the hepatoprotective effects of bees’ honey on rats, the experiment was carried out on four groups of female Wister rats. Hepatic lesions were induced by intraperitoneal (i.p) injection of carbon tetrachloride (0.5 ml/Kg body weight (b.wt)) diluted in olive oil (0.5 ml/Kg with 1:1 ratio), twice a week a period of for four weeks. Biochemical parameters of hepatic damage such as serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and catalase (CAT) activity were determined. Four weeks treatment using Carbon tetrachloride increased the serum AST, ALT and LDH levels significantly compared to control group (G1), while these enzymes were significantly reduced by the effect of honey (10%) plus CCl4 (0.5ml/Kg (b.wt) (i.p.)) for 4 weeks compared to CCl4 group (G3) (P< 0.05), but there were significant decreases in catalase (CAT) values of CCl4 group (G3) compared to the control group (G1) (P< 0.05).

Keywords: Liver, hepatotoxic , Free radical, Antioxidation.

المراجع References

[1]. Loguercio C, Federico A (2003) Oxidative stress in viral and alcoholic hepatitis. Free Radical Biol Med. 34:1–10.
[2]. Vitaglione P, Morisco F, Caporaso N, Fogliano V (2004) Dietary antioxidant compounds and liver health. Crit Rev Food Sci Nutr 44:575–586.
[3]. Nan JX, Jiang YZ, Park EJ, Ko G, Kim YC, Sohm DH (2003) Protective effect of Rhodiola sachalinesis extract on carbon tetrachloride induced liver injury in rats. J Ethnopharmacol. 84:143–148.
[4]. Lee TY, Mai LM, Wang GJ, Chiu JH, Lin YL, Lin HC (2003) Protective mechanism of Salvia miltiorrhiza on carbon tetrachloride induced hepatotoxicity in rats. J Pharmacol Sci. 91:202–210.
[5]. Pradeep HA (2009) Hepatoprotective evaluation of Anogeissus latifolia : In vitro and in vivo studies. World J. Gastroenterol. 15(38):4816-22.
[6]. Wagner HK, Wolff PM (2011) New Natural Products and Plant Drugs with Pharmacological, Biological Or Therapeutical Activity: Proceedings of the First International Congress on Medicinal Plant Research, Section A, Held at the University of Munich, Germany, September 6-10, 1976. Springer London, Limited.
[7]. Kim H.Y, Kim J.K, Choi J.H, Jung J.Y, Oh W.Y, Kim D.C, Lee H.S, Kim Y.S, Kang S.S, Lee S.H, and Lee S.M (2010) Hepatoprotective effect of pinoresinol on carbon tetrachloride-induced hepatic damage in mice. J. Pharmacol. Sci. 112(1):105-112.
[8]. Ohta Y, Nishida K, Sasaki E, Kongo M, Ishiguro I (1999) Attenuation of disrupted hepatic active oxygen metabolism with the recovery of acute liver injury in rats intoxicated with carbon tetrachloride. Res Commun Mol Pathol Pharmacol. 95:191–207.
[9]. Ozturk F, Ucar M, Ozturk IC, Vardi N, Batcioglu K (2003) Carbon tetra-chloride induced nephrotoxicity and protective effect of betaine in Sprague-Dawley rats. Urology. 62:353–356.
[10]. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J (2007) Free radicals and antioxidantsin normal physiological functions and human disease. Int J Biochem Cell Biol. 39:44–84.
[11]. Alvarez-Suarez, J. M., Tulipani, S., Bertoli, S. R.-E., & Battino, M (2010) Contribution of honey in nutrition and human health: a review. Mediterranean Journal of Nutrition and Metabolism. 3, 15-23.
[12]. Gheldof, N., & Engeseth, N. J (2002) Antioxidant capacity of honeys from various floral sources based on the determination of oxygen radical absorbance capacity and inhibition of in vitro lipoprotein oxidation in human serum samples. Journal of Agricultural and Food Chemistry. 50, 3050-3055.
[13]. Sato, T., & Miyata, G (2000) The Nutraceutical Benefit, Part III: Honey. Nutrition 16, 468-469.
[14]. Molan, P. C (2001) Why honey is effective as a medicine. 2. The scientific explanation of its effects. Bee World. 82(1), 22-40.
[15]. Molan, P. C (2009) Why honey works. In R. Cooper, P. Molan & R. White (Eds.), Honey in modern wound management. Aberdeen: Wounds UK Ltd.
[16]. Aljadi, A., & Kamaruddin, M (2004) Evaluation of the phenolic contents and antioxidant capacities of two Malaysian floral honeys. Food Chemistry. 85(4), 513-518.
[17]. Nasuti, C., Gabbianelli, R., Falcioni, G., & Cantalamess, F (2006) Antioxidative and gastroprotective activities of anti-inflammatory formulations derived from chestnut honey in rats. Nutrition Research. 26, 130-137.
[18]. Schramm, D. D., Karim, M., Schrader, H. R., Holt, R. R., Cardetti, M., & Keen, C. L (2003) Honey with high level of antioxidants can provide protection to healthy human subjects. Journal of Agricultural and Food Chemistry. 51, 1732-1735.
[19]. Vela, L., de Lorenzo, C., & Pérez, R. A (2007) Antioxidant capacity of Spanish honeys and its correlation with polyphenol content and other physicochemical properties. Journal of the Science of Food and Agriculture. 87(6), 1069-1075.
[20]. Karlsson, J (1997) Introduction to Nutraology and Radical Formation. In J. Karlsson (Eds), Antioxidants and Exercise (pp. 1-143). Illinois: Human Kinetics Press.
[21]. Cheeseman, K. H., & Slater, T. F (1993) An introduction to free radical biochemistry. British Medical Bulletin. 49, 481-493.
[22]. Cochrane, C. G (1991) Cellular injury by oxidants. American Journal of Medicine. 91(Suppl. 3c), 23S-30S.
[23]. Halliwell, B., & Gutteridge, J (1989) Free Radicals in Biology and Medicine.
Oxford, UK: Calendon Press.
[24]. Banskota A.H, Tezuka Y, and Kadota S (2001) Recent progress in pharmacological research of propolis. Phytotherapy Res.15:561-571.
[25]. Macías F.A, Oliveiros-Bastidas A, Marín D, Carrera C, Chinchilla N, and Molinillo J.M.G ( 2008) Plant biocommunicators: their phytotoxicity, degradation studies and potencial use as herbicides models. Phytochem. Rev. 7: 179–194.
[26]. Akram, E; Pejman, M; Mayam, B; Jalal, Z (2012) Hepatoprotective activity of cinnamon ethanolic extract against CCl4-induced liver injury in rats. EXCLI journal.11: 495-507.
[27]. Hong R .T, Xu J.M, and Mei Q (2009) Melatonin ameliorates experimental hepatic fibrosis induced by carbon tetrachloride in rats. World J. Gastroenterol. 15(12): 1452-1458.
[28]. Fang Y-Z, Yang S, Wu G (2002) Free radicals, antioxidants, and nutrition. Nutrition.18(10):872–9.
[29]. Almalki A. L (2013) “Oat attenuation of hyperglycemia-induced retinal oxidative stress and NF-𝜅B activation in streptozotocininduced diabetic rats,” Evidence-Based Complementary and AlternativeMedicine, vol. 2013, Article ID 983923. 8 pages.
[30]. Ulican O, Greksak M, Vancova O, Zlatos L, Galbavy S, Bozek P, Nakano M (2003) Hepatoprotective effect of Rooibos tea (Aspalathus linearis) on CCl4 –induced liver damage in rats. Physiol. Res. 52: 461-466.
[31]. Adebajo A.C, Iwalewa E.O, Obuotor E.M, Ibikunle G.F, Omisore N.O, and Adewunmi C.O (2009) Pharmacological properties of the extract and some isolated compounds of Clausena lansium stem bark: anti-trichomonal, antidiabetic, anti-inflammatory, hepatoprotective and antioxidant effects. J Ethnopharmacol.122 (1):10–9.
[32]. Jaramillo-Juarez F, Rodriguez-Vazquez M.L, Rincon-Sanchez A.R, Consolacion M, Martinez G.G, Ortiz J, and Reyes JL (2008) Caffeic acid phenethyl ester against carbon tetrachloride toxicity in rats. Ann Hepatol. 7:331–338.
[33]. Resende R.J, Beletti M.E, Machado E.R, Fonseca N.M and Mundim A.V (2003) Effect of honey and amino acids containing either levulose or liver proteolysates against carbon tetrachloride-induced acute hepatotoxicity in mice. Arch. Velerinaria. 19: 104-109.
[34]. Beretta G, Granata P, Ferrero M, Orioli M, and Facino M.R (2005) Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. Anal Chim Acta.533:185–91.